Bacterial Colonization of Tumors Drives Immune Activation and Checkpoint Blockade Efficacy

Using 16S rRNA qPCR, sequencing, and culture-based methods on multiple murine tumor models, we identified intratumoral microbiota in MCA-205 tumors but not in MOC1 tumors, underscoring the role of tumor-intrinsic factors in shaping microbial colonization.

我们针对多种小鼠肿瘤模型（murine tumor models），采用16S核糖体RNA定量聚合酶链反应（16S rRNA qPCR）、测序以及培养法开展研究，在MCA-205肿瘤中鉴定出瘤内微生物群（intratumoral microbiota），但未在MOC1肿瘤中检测到该菌群，这突显了肿瘤内在因素（tumor-intrinsic factors）在塑造微生物定植（microbial colonization）过程中的作用。