Pan-cancer analysis of pyrimidine metabolism using pathway-based approach unravels complex signaling pathways connections with a role in chemoresistance

To investigate the effect of DHODH inhibitor, Brequinar (BRQ), in inhibiting the pyrimidine metabolism and its associated signaling pathways, lung cancer cell line (A549) was treated with BRQ for 72 hours and performed gene expression profiling using RNA-seq. Overall design: Differential gene expression analysis for A549 cells treated with BRQ and control cells at 72 hours in replicates.

为探究二氢乳清酸脱氢酶（DHODH）抑制剂布喹那（Brequinar, BRQ）对嘧啶代谢及其相关信号通路的抑制作用，本研究将肺癌细胞系A549经BRQ处理72小时后，采用RNA测序（RNA-seq）开展基因表达谱分析。实验设计：对经BRQ处理72小时的A549细胞与对照组细胞进行重复样本的差异基因表达分析。