RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector. RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

In order to investigated the underlying molecular mechanism of GR’s effects, we transfected the Min6 cell with the GR cDNA expression vector or control vector, and then extracted RNA of Min6 cells for RNA sequencing. We studied the gene-expression differences resulting in GR overexpress. A total of 21542 gene transcripts was captured,and 233 genes showed significant expression differences (p-value1) between the two group. Further GO analysis and KEGG analysis found that GR overactivation instigates dysregulated autophagy in beta-cells Overall design: mRNA of Min6 cells transfected Control vector or GR expression vector

为探究糖皮质激素受体（GR）发挥作用的潜在分子机制，我们将Min6细胞分别转染GR cDNA表达载体与对照载体，随后提取Min6细胞的总RNA进行RNA测序（RNA sequencing），以此分析糖皮质激素受体过表达所引发的基因表达差异。本研究共捕获21542条基因转录本，两组间共有233个基因呈现出显著的表达差异（p-value1）。进一步的基因本体（GO，Gene Ontology）富集分析与京都基因与基因组百科全书（KEGG，Kyoto Encyclopedia of Genes and Genomes）通路分析结果显示，糖皮质激素受体过度激活会诱导胰岛β细胞发生自噬功能紊乱。实验整体设计：转染对照载体或GR表达载体的Min6细胞的mRNA。