Genome-wide titration of gene expression in M. tuberculosis and M. smegmatis

Genome-wide CRISPRi libraries in M. tuberculosis H37Rv, M. tuberculosis HN878, and M. smegmatis mc2-155 were grown in the presence and absence of ATc to induce CRISPR interference. Titration of expression was achieved through the use of single-guide RNAs of different efficiency. Libraries were sequenced at approximately 0, 2.5, 5, 7.5, 10, 15, 20, 25 and 30 generations of cumulative growth.

本数据集所涉及的全基因组CRISPR干扰（CRISPR interference, CRISPRi）文库分别构建于结核分枝杆菌（Mycobacterium tuberculosis）H37Rv、结核分枝杆菌（Mycobacterium tuberculosis）HN878以及耻垢分枝杆菌（Mycobacterium smegmatis）mc²-155菌株中。实验过程中，将携带上述文库的菌株在添加ATc与不添加ATc的培养条件下培养，以诱导CRISPR干扰；通过使用不同敲降效率的单引导RNA（single-guide RNA, sgRNA），实现了基因表达水平的梯度滴定。最终，分别在菌株累计生长至约0、2.5、5、7.5、10、15、20、25及30代时，对各文库进行测序。