CIL:34251, Homo sapiens, endocrine-paracrine cell of prostate gland, basal cell of prostate epithelium, luminal cell of prostate epithelium, blood vessel endothelial cell, perineural cell, sheath cell, leukocyte, prostate stromal cell, smooth muscle cell of prostate. In Cell Image Library

Preparation: acetone fixed tissue; tissue in polyethylene glycol embedment
Relation to intact cell: cryostat-sectioned tissue
Item type: recorded image
Imaging mode: widefield illumination; bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein; differences in intrinsic optical density
Visualization methods: hematoxylin; primary antibody plus labeled secondary antibody; horseradish peroxidase; 3,3'-diaminobenzidine (DAB); biotin-avidin amplification
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

样本制备：丙酮（acetone）固定组织，以聚乙二醇（polyethylene glycol）包埋组织；与完整细胞的对应关系：冷冻切片组织（cryostat-sectioned tissue）；数据条目类型：成像记录；成像模式：宽场照明（widefield illumination）；明场显微镜成像（bright-field microscopy）；成像参数：照明光吸光度（absorption of illumination）；对比度来源：染色剂吸附或结合差异、特定蛋白质的分布特征、固有光密度差异；可视化方法：苏木精（hematoxylin）、一抗加标记二抗体系、辣根过氧化物酶（horseradish peroxidase）、3,3'-二氨基联苯胺（3,3'-diaminobenzidine，DAB）、生物素-亲和素扩增法（biotin-avidin amplification）；处理历史：未处理原始数据；数据属性：原始数据、强度定量