Paternal starvation affects metabolic gene expression during zebrafish offspring development and life-long fitness

Dietary restriction in the form of fasting is a putative key to a healthier and longer life, but these benefits may come at a trade-off with reproductive fitness and may affect the following generation(s). The potential inter- and transgenerational effects of long-term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split-egg clutch design to explore the male-specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected, we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hours post fertilisation in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fat...,
Animal model 

We used wild-type Zebrafish Danio rerio from the AB strain obtained from ZIRC (Zebrafish International Resource Center, University of Oregon, Eugene, USA) and maintained at the SciLifeLab zebrafish platform at Uppsala University (http://www.scilifelab.se/facilities/zebrafish/) and the Controlled Ecology Facility (CEF) at the University of East Anglia (UEA, UK). The fish were kept in 3L tanks in a recirculating rack system (Aquatic Habitats (Uppsala) and Techniplast (UEA)) at 26.4 ± 1.4°C and a 12:12 diurnal light cycle. 

Feeding regime 

Fish were fed three times a day with a mixture of dry pellets and live artemia. For the experiments, 10-16 males were randomly split into 3L tanks assigned to the two experimental groups, referred to as Control and Starved. Each male was assigned an ID number and weighed and imaged for posterior identification through pigmentation and fin shape features. Males were maintained with wild-type companion females at a total..., , # Paternal starvation affects metabolic gene expression during zebrafish offspring development and life-long fitness

[https://doi.org/10.5061/dryad.ncjsxkt2j](https://doi.org/10.5061/dryad.ncjsxkt2j)

These datasets contain the data collected during the phenotypic analysis of males, F1, and F2 generations in our study.

## Description of the data and file structure

### **CSV File Name: F1_survival2months_sexratio.txt**

Description of file: Data collected upon analysis of the F1 survival at 2 months postfertilization and their sex ratios

*Variables:*

* IVF number: In vitro fertilization (IVF) replicate number
* female.ID: Identity of female used in IVF
* male.ID: Identity of male used in IVF
* Treatment: Male feeding regime (Fed or Starved)
* total number offspring: Number of embryos produced in a round of IVF
* survival number offspring: Number of surviving embryos after IVF
* number males: Number of males in F1 offspring at 2 months post fertilization

### **CSV File Name: F1_yolk...,

以禁食形式实施的膳食限制，被认为是助力健康长寿的关键途径，但此类益处可能会以牺牲繁殖适合度为代价，并对子代产生影响。长期禁食与饥饿所引发的跨代及跨世代效应，在源自父系的脊椎动物中尤其未被充分阐明。本研究利用可支持分卵批次实验设计的体外受精斑马鱼模型，在独立于母本贡献的前提下，探究禁食/饥饿对雄性生育能力以及子代适合度的特异性影响。为期18天的禁食使受试雄性的生育能力下降。尽管子代的平均存活率未受影响，但我们观察到，饥饿组雄性产生的F1子代幼体生长速率提升，而由饥饿组雄性的F1子代所繁育的F2子代中，受精后24小时的胚胎畸形率显著升高。本研究还对饥饿组与喂食组雄性所产生的F1胚胎的转录组进行了比较…… 动物模型

## 动物模型
本研究使用的AB品系野生型斑马鱼（Danio rerio）购自美国俄勒冈大学尤金分校斑马鱼国际资源中心（ZIRC, Zebrafish International Resource Center, University of Oregon, Eugene, USA），并分别在乌普萨拉大学SciLifeLab斑马鱼平台（http://www.scilifelab.se/facilities/zebrafish/）以及英国东英吉利大学受控生态设施（CEF, Controlled Ecology Facility, University of East Anglia, UK）中饲养。实验鱼饲养于3升水箱中，采用循环养殖架系统（乌普萨拉分校使用Aquatic Habitats系统，东英吉利大学使用Techniplast系统），饲养温度维持在26.4±1.4℃，光周期为12小时光照:12小时黑暗。

## 喂食方案
实验鱼每日投喂三次，投喂饲料为干饲料颗粒与活体卤虫的混合饲料。实验阶段，将10~16尾雄性斑马鱼随机分配至3升水箱，分为对照组（Control）与饥饿组（Starved）两个实验组。每尾雄性斑马鱼均被赋予唯一ID，并进行称重与成像，后续可通过体色与鳍形特征进行个体识别。雄性斑马鱼与野生型伴养雌性共同饲养，总……

# 父系饥饿影响斑马鱼子代发育过程中的代谢基因表达与终生适合度
DOI: 10.5061/dryad.ncjsxkt2j
本数据集包含本研究中对雄性亲本、F1代与F2代进行表型分析所收集的全部数据。

## 数据与文件结构说明
### CSV文件名：F1_survival2months_sexratio.txt
文件说明：该文件包含受精后2个月时F1代存活率及其性别比例的分析数据
*变量说明：*
* IVF编号：体外受精（IVF, In vitro fertilization）重复实验编号
* 雌性ID：体外受精所用雌性斑马鱼的标识
* 雄性ID：体外受精所用雄性斑马鱼的标识
* 处理组：雄性斑马鱼的喂食方案（喂食组或饥饿组）
* 总子代数：单次体外受精所产生的胚胎总数
* 存活子代数：体外受精后存活的胚胎数
* 雄性子代数：受精后2个月时F1代子代中的雄性个体数

### CSV文件名：F1_yolk……