Comparison of the tarnscriptome of 4T1 control cells versus shRNA NFAT1 transduced cells (mouse)

4T1 is a mammary tumor cell line to which the NFAT1 transcription factor is essential for tumorigenesis and metastasis. control or shRNA transduced 4T1 cells growing in culture were collected and RNA was extracted from each sample and processed for hybridization to Affymetrix arrays.

4T1是一种乳腺肿瘤细胞系，NFAT1转录因子对其成瘤与转移过程至关重要。研究人员收集了体外培养的经对照处理或短发夹RNA（shRNA）转导的4T1细胞，从每份样本中提取RNA，经样本预处理后与Affymetrix基因芯片进行杂交实验。