Translation efficiency is maintained during heat shock in Escherichia coli. Escherichia coli str. K-12 substr. MG1655

We utilize ribosome profiling to directly monitor translation in E. coli at 30 °C and investigate how this changes after 10-20 minutes of heat shock at 42 °C. Translation is controlled by the interplay of several RNA hybridization processes, which are expected to be temperature sensitive. We observe that translation efficiencies are robustly maintained after thermal heat shock and after mimicking the heat shock response transcriptional program at 30 °C. Overall design: Ribosome profiling and RNA-seq analysis of E. coli was used to measure translation efficiency. The effect of heat shock was measured under 3 conditions (n=3); overexpression of wildtype (n=3) or constitutively active mutant (n=2) heat shock transcription factor rpoH was compared to cells expressing empty vector (n=4).

本研究采用核糖体谱（ribosome profiling）技术，直接监测30 ℃条件下大肠杆菌（E. coli）的翻译过程，并探究其在42 ℃热激10~20分钟后的变化规律。翻译过程受多种RNA杂交过程的协同调控，此类过程理论上具有温度敏感性。本研究发现，无论是经42 ℃热激处理，还是在30 ℃下模拟热激应答转录程序，大肠杆菌的翻译效率均能得到稳健维持。实验整体设计：通过对大肠杆菌开展核糖体谱与RNA测序（RNA-seq）分析，定量测定其翻译效率。热激效应的检测共设置3组实验条件（每组n=3）；将过表达野生型热激转录因子rpoH（n=3）或组成型活性突变型rpoH（n=2）的菌株，与转染空载体的对照菌株（n=4）进行对照比较。