Examples of genotype-by-sequencing (GBS) methods using restriction enzymes and high throughput DNA sequencing to select, sequence and genotype a large set of loci across multiple samples.

*Using SbfI enzyme.**Using “narrow” size range (see [6]).***Using selective adapters.To facilitate comparison, the expected number for each method is estimated for the zebra finch genome based on the specific restriction enzymes and other parameters (e.g., size selection) used in each study.Examples of genotype-by-sequencing (GBS) methods using restriction enzymes and high throughput DNA sequencing to select, sequence and genotype a large set of loci across multiple samples.

- 使用SbfI限制性内切酶（SbfI enzyme）
- 采用“窄幅”片段大小筛选范围（详见参考文献[6]）
- 使用选择性接头（selective adapters）

为便于跨研究间的横向对比，本研究针对各研究所使用的特定限制性内切酶及其他参数（如片段大小筛选步骤），基于斑胸草雀基因组估算了每种方法的预期位点数量。

以下示例展示了一类结合限制性内切酶与高通量DNA测序技术的基因型测序（genotype-by-sequencing, GBS）方法，可在多个样本中筛选、测序并完成大量基因组位点的基因分型。