Bulk RNAseq Analysis of Murine Thoracic Aortas with Tsc1+/+ and Tsc1-/- Smooth Muscle Cells. Bulk RNAseq Analysis of Murine Thoracic Aortas with Tsc1+/+ and Tsc1-/- Smooth Muscle Cells

We characterized the transcriptomic effects of chronic mTOR activation in aortic SMCs using a conditional genetic model to delete Tsc1 under control of a smooth muscle-specific Myh11 promoter Overall design: Myh11-CreERT2.mT/mG (Tsc1+/+) and Tsc1fl/fl.Myh11-CreERT2.mT/mG (Tsc1−/−) male mice were treated with tamoxifen at 1.5 wk and the thoracic aortas were analyzed by bulk RNAseq at 24 wk.

本研究采用以平滑肌特异性Myh11启动子为调控元件的条件性遗传模型敲除Tsc1基因，以表征主动脉平滑肌细胞（smooth muscle cells, SMCs）中慢性mTOR（哺乳动物雷帕霉素靶蛋白，mammalian target of rapamycin）激活的转录组学效应。实验整体设计：将Myh11-CreERT2.mT/mG（Tsc1+/+，Tsc1野生型）与Tsc1fl/fl.Myh11-CreERT2.mT/mG（Tsc1−/−，Tsc1纯合敲除型）雄性小鼠于1.5周龄时给予他莫昔芬诱导处理，并在24周龄时通过批量RNA测序（bulk RNA-seq）分析其胸主动脉组织。