Transcriptional Responses to Elevated Temperature in Streptococcus agalactiae

In the transition from recto-vaginal colonizing organism to invasive pathogen, Streptococcus agalactiae (Group B Streptococcus, GBS) must adapt to changes in host temperature, including elevated temperatures due to host fever. To identify genes important to the survival of GBS in response to heat stress, transcriptional profiling was performed using DNA microarray analysis, comparing GBS grown at normal temperature (37˚C) to GBS exposed to elevated temperature (42˚C). Two separate RNA samples were extracted for each condition. Three hybridizations were conducted total.

无乳链球菌（Streptococcus agalactiae，Group B Streptococcus，GBS）从直肠阴道定植菌转变为侵袭性病原菌的过程中，需适应宿主体温的变化，包括宿主发热导致的体温升高。为鉴定参与无乳链球菌应对热应激生存过程的关键基因，研究人员采用DNA微阵列分析（DNA microarray analysis）开展转录谱分析（transcriptional profiling），对比了在正常体温（37℃）下培养的无乳链球菌与暴露于高温（42℃）环境的无乳链球菌的转录表达谱。针对两种培养条件，各独立提取两份核糖核酸（RNA）样本，最终共完成三次杂交实验。