DBC1 is a positive regulator of enhancer epigenomic writers KMT2D and p300 [HCT116 RNA-seq]

We identified DBC1/CCAR2 target genes using RNA-seq analysis in colon cancer cells. Overall design: mRNA profiles of HCT116 WT or DBC1 KO cells were generated by deep sequencing, in triplicate, using illumina NovaSeq6000.

本研究在结肠癌细胞中通过RNA测序（RNA-seq）分析鉴定了DBC1/CCAR2的靶基因。整体实验设计：采用Illumina NovaSeq6000测序平台对HCT116野生型（WT）或DBC1敲除（KO）细胞的mRNA表达谱进行深度测序，每组设置三次生物学重复。