Shotgun and Capture data from sedaDNA from North Siberia (lake CH12) using the Larix chloroplast genome

We extracted DNA from a lake sediment core (130cm) from northern Siberia, Taymyr region, lake CH12 (72.399?N, 102.289?E). We did shotgun sequencing on 4 samples along the core 6700 calibrated years BP, 5400 c.y.BP, 1900 c.y.BP and 60 c.y.BP together with the extraction blank and a library blank. On the same sample set we did hyridization capture with long-range PCR generated baits covering the complete chloroplast genome of Larix gmelinii. We sequenced both project on a Illumina HiSeq2500 with 125bp-PE sequencing.

我们从西伯利亚北部泰梅尔（Taymyr）地区CH12湖（坐标72.399°N，102.289°E）的130厘米湖相沉积岩芯中提取了脱氧核糖核酸（DNA）。我们对该岩芯的4个年代样品（分别为校准距今6700年、5400年、1900年及60年），以及提取空白对照与文库空白对照，开展了鸟枪法测序（shotgun sequencing）。我们采用长程聚合酶链式反应（long-range PCR）制备的覆盖兴安落叶松（Larix gmelinii）完整叶绿体基因组的诱饵探针，对同一样品组进行了杂交捕获（hybridization capture）。本研究的两个测序项目均使用Illumina HiSeq2500测序平台完成125bp双端（125bp-PE）测序。