S. cerevisiae RNA-seq in wild-type and sir2Δhst1Δ strains

RNA-seq to identify differentially expressed genes in the sir2Δhst1Δ strain compared to wild-type The sequenced strains are matα with hmr deleted to avoid pseudodiploid conditions. Differential expression in the sir2Δhst1Δ strain was used to avoid any complementation in a single deletion strain. This experiment represents two technical replicates for each strain

本研究采用RNA测序（RNA-seq）技术，鉴定sir2Δhst1Δ菌株与野生型（wild-type）菌株间的差异表达基因。本次测序的菌株为携带hmr基因缺失的matα菌株，以避免假二倍体（pseudodiploid）状态。本研究选取sir2Δhst1Δ双缺失菌株进行差异表达分析，以规避单基因缺失菌株中可能存在的基因互补效应。本实验为每种菌株设置了两次技术重复（technical replicates）。