pcos2017

Research hypothesis: Administration of aqueous extract of Agnus castus leaves to pcos-induced female Wistar rats is beneficial
What data shows: the current data shows the administrations of Agnus castus at all doses administered led to an increase in the activities of Alkaline Phosphatase, catalase, and superoxide dismutase from 1357.32 nM/min/mg, 0.020 U/mg protein and 361.11 U/mg to 2230.64 nM/min/mg, 0.10 U/mg protein, and 583.33 U/mg protein respectively. Also, glucose (175 mg/100mg to 691.67 mg/100mg) and cholesterol levels (173.77 mg/dl to 182.81 mg/dl) increased when compared with the letrozole administered rats. There was a slight depletion in the levels of uterine protein (from 1.23mg/g to 1.13mg/g). Serum levels of testosterone increased in letrozole administered rats but decreased in animals that received Agnus castus extracts. Follicle stimulating hormone and estradiol level increased in animals administered with Agnus castus compared with letrozole administered animals.
Notable findings:
- Elevated testosterone level was reduced in Agnus castus treated rats
- Lipid peroxidation levels reduced considerably in Agnus castus treated rats
- Reduced level of antioxidant enzymes was augmented after Agnus castus treatment
How the data can be interpreted: Although Agnus castus aqueous leaves extract seemed to show some beneficial effects on letrozole-administered pcos animals, the changes observed were not significant.

研究假说：向由多囊卵巢综合征（Polycystic Ovary Syndrome, PCOS）诱导的雌性Wistar大鼠灌服穗花牡荆（Agnus castus）叶片水提物具有积极作用。数据结果表明：本次研究数据显示，给予不同剂量穗花牡荆（Agnus castus）后，碱性磷酸酶（Alkaline Phosphatase）、过氧化氢酶（catalase）及超氧化物歧化酶（superoxide dismutase）的活性分别从1357.32 nM/min/mg、0.020 U/mg蛋白及361.11 U/mg提升至2230.64 nM/min/mg、0.10 U/mg蛋白及583.33 U/mg蛋白。此外，与来曲唑（letrozole）处理组大鼠相比，给药组大鼠的血糖水平（175 mg/100mg 升至 691.67 mg/100mg）及胆固醇水平（173.77 mg/dl 升至 182.81 mg/dl）均有所升高。同时，子宫蛋白质水平存在小幅下降（从1.23mg/g 降至1.13mg/g）。来曲唑处理组大鼠的血清睾酮水平升高，但给予穗花牡荆提取物的大鼠血清睾酮水平则有所降低。相较于来曲唑处理组，穗花牡荆给药组大鼠的促卵泡生成素（Follicle stimulating hormone）及雌二醇（estradiol）水平均有所升高。核心研究发现：- 穗花牡荆处理组大鼠原本升高的睾酮水平显著降低；- 穗花牡荆处理组大鼠的脂质过氧化（lipid peroxidation）水平大幅降低；- 经穗花牡荆治疗后，抗氧化酶活性低下的状况得到显著改善。数据解读：尽管穗花牡荆叶片水提物在多囊卵巢综合征模型大鼠中展现出一定的获益潜力，但本次观测到的各项变化均未达到统计学显著性水平。