Transcriptome profile of fibroblast-like synoviocytes with FTO knockdown

Fibroblast-like synoviocytes (FLS) are crucial in promoting articular inflammation and destruction in rheumatoid arthritis (RA). As the most abundant RNA modification, the function of m6A in RA FLS is still unclear. Here, we constructed FTO-knockdown FLS to explore the mechanism of FTO in regulating the aggressive behavior of RA FLS. To investigate the function of FTO in RA FLS，FTO-knockdown FLS were established by shRNA-lentivirus infection. RNA sequencing was then performed to explore the specific downstream targets of FTO.

成纤维样滑膜细胞（Fibroblast-like synoviocytes, FLS）在类风湿关节炎（Rheumatoid arthritis, RA）的关节炎症发生与组织破坏进程中发挥关键作用。作为体内丰度最高的RNA修饰类型，N6-甲基腺苷（m6A）在RA FLS中的功能仍未明确。本研究构建了FTO敲低的RA FLS，以探究FTO调控RA FLS侵袭行为的分子机制。为明确FTO在RA FLS中的功能，我们通过短发夹RNA慢病毒（shRNA-lentivirus）感染成功构建FTO敲低的RA FLS细胞模型，随后开展RNA测序以筛选FTO的特异性下游靶标。