High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [GM12878]. High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [GM12878]

While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages. Overall design: Variable bead concentrations and loading conditions for testing species mixing and data quality from SureCell scATAC-seq on GM12878 cells

尽管近年来技术进步已推动实现单细胞分辨率下的表观基因组图谱绘制，但现有方法的通量与质量仍限制了此类技术的大规模应用。本研究介绍一种基于液滴微流控技术的高通量单细胞转座酶可及性染色质测序（single-cell assay for transposase accessible chromatin, scATAC-seq）分析平台，该平台具备高通量图谱分析所需的核心性能，包括提升单细胞文库复杂度与测序通量。该方法可实现复杂组织的可靠细胞分型，并构建出涵盖多种细胞类型与细胞阶段的全新细胞及表观基因组多样性图谱。实验整体设计：针对GM12878细胞开展SureCell scATAC-seq实验时，通过调整磁珠浓度与上样条件，以测试物种混合效果与数据质量。