Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China

Abstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.

摘要：遗传多样性匮乏是制约我国陆地棉遗传改良的关键瓶颈，因此亟需开发新技术，以从现有育种资源中挖掘更多与农艺性状相关的多态性分子标记。本研究建立了一套结合自动化片段分析仪ABI3500xl的序列相关扩增多态性（sequence-related amplified polymorphism, SRAP）分子标记高效经济技术体系，用于检测陆地棉的遗传多样性。借助该技术策略，研究团队可便捷地对7872对SRAP引物开展多态性筛选，共检测到504个多态性标记。其中选取165个标记，对国内收集的128份陆地棉品种进行遗传多样性分析。本方法将传统分子标记开发技术与经济简便的操作策略相结合，可为育种工作者提供实用的技术支撑。