RNA-seq analysis of control and MCUR1-depleted HSCs

We sought to explore the underlying mechanisms by which MCUR1 promotes erythropoiesis. Total RNAs were extracted from cultured human CD34+ HPCs stably expressing sh-MCUR1 or sh-Ctrl under hypoxia (1% O2) following 2 days of EPO stimulation, using TRIZOL Reagent (Invitrogen, CA, USA). RNA sequencing (RNA-seq) was performed using Illumina NovaSeq6000 at Berry Genomics Co. Ltd. (Beijing, China), and a mean of 40 million paired-end reads per sample was obtained.

本研究旨在探究MCUR1促进红细胞生成的潜在分子机制。本研究从已培养的、稳定表达靶向MCUR1的短发夹RNA（sh-MCUR1）或阴性对照短发夹RNA（sh-Ctrl）的人CD34阳性造血祖细胞（HPCs）中提取总RNA：将上述细胞经促红细胞生成素（EPO）刺激2天后，置于低氧（1% O₂）环境中培养，提取过程使用TRIZOL试剂（Invitrogen，美国加利福尼亚州）。本研究采用Illumina NovaSeq6000测序平台，于中国北京的Berry Genomics Co. Ltd.完成RNA测序（RNA-seq），每个样本平均获得4000万条双端测序读段（reads）。