Shaping of Tumor and Drug Resistant Genomes by Instability and Selection. Homo sapiens

We used genome-wide microarray comparative genomic hybridization to carry out a higher resolution evaluation of the effect of MMR competence on genomic alterations occurring in 20 cell lines and to determine if characteristic aberrations arise in MMR proficient and deficient HCT116 cells undergoing selection for methotrexate resistance. Keywords: Comparative Genomic Hybridization Overall design: Array CGH experiments of cell line genomic DNA as test samples and normal human genomic DNA as reference sample.

本研究采用全基因组微阵列比较基因组杂交（genome-wide microarray comparative genomic hybridization）技术，对20株细胞系中发生的基因组变异受错配修复（MMR，Mismatch Repair）功能完整性的影响开展高分辨率评估，并探究经甲氨蝶呤抗性筛选的错配修复功能正常与缺陷型HCT116细胞是否会出现特征性染色体畸变。
关键词：比较基因组杂交（Comparative Genomic Hybridization）
实验设计：以细胞系基因组DNA作为测试样本、正常人类基因组DNA作为参照样本的阵列比较基因组杂交（Array CGH）实验。