Raw sequence reads of a cell aggregate comprising of anaerobic methanotrophic archaea and Limnobacter related to betaproteobacteria

Anaerobic methanotrophic Archaea (ANME) have been observed forming cell aggregates with Betaproteobacteria; however, the metabolic potentials and roles of these Betaproteobacteria in the communities that perform an anaerobic oxidation of methane (AOM) are not yet understood. A cell aggregate that contained Limnobacter, which belongs to the Betaproteobacteria, and ANME2a was captured by micromanipulation. To obtain the genomic information, this aggregate was subject to multiple displacement amplification and Illumina Hiseq sequencing.

厌氧产甲烷古菌（Anaerobic methanotrophic Archaea, ANME）已被观测到可与β-变形菌门（Betaproteobacteria）形成细胞聚集体；然而，这类β-变形菌门微生物在执行甲烷厌氧氧化（anaerobic oxidation of methane, AOM）的群落中所具备的代谢潜能与生态功能，目前仍未得到阐明。研究人员通过显微操纵术（micromanipulation）捕获了一株同时包含湖杆菌属（Limnobacter，隶属于β-变形菌门）与ANME2a的细胞聚集体。为获取该聚集体的基因组信息，研究人员对其开展了多重置换扩增（multiple displacement amplification）与Illumina Hiseq测序。