Expression data from CD19-positive splenic B cells isolated from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice. Mus musculus

The function of ID4 in CLL development was studied in vivo using TCL1 transgenic mouse model that develop leukemia similar to human CLL. TCL1 mice with ID4 single knockout gene have accelerated CLL progression. Results from the animal study suggest ID4 as a tumor suppressor gene that might regulate cell proliferation and apoptosis in B lymphocytes. Overall design: Gene expression in CD19-positive splenic B cells collected from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice was compared by microarray, the goal is to find ID4-regulated genes involved in CLL development.

本研究采用可模拟人类慢性淋巴细胞白血病（Chronic Lymphocytic Leukemia, CLL）发病进程的TCL1转基因小鼠模型，在体内探究ID4在CLL发生发展中的功能。携带ID4单基因敲除的TCL1小鼠，其CLL疾病进展显著加快。该动物实验结果提示，ID4可作为抑癌基因，调控B淋巴细胞的细胞增殖与凋亡过程。整体实验设计：通过微阵列（microarray）技术对比1月龄ID4杂合敲除（ID4+/-TCL1-tg）与ID4野生型（ID4+/+TCL1-tg）小鼠的CD19阳性脾脏B细胞的基因表达水平，旨在筛选参与CLL发生发展的ID4调控靶基因。