Relative pigment content in intact cells of R. salina and in their chlorophyll a/c2 antennae (CAC antennae).

Intact cells were adapted either to dark (“Cells – dark”) or exposed to intense irradiation (1500 µmol m−2 s−1) for 1 hour (“Cells – light”). CAC antennae were isolated by sucrose gradient and fraction 1 was used for analysis (see Figure 5A). Carotenoids were extracted to methanol and separated by HPLC. Note, that data represent a peak area of particular pigments relative to chlorophyll a. The total carotenoid and Chl a level was not changed significantly after high light stress.

完整细胞分别适应黑暗环境（记为"Cells – dark"，即黑暗组细胞），或置于强度为1500 µmol·m⁻²·s⁻¹的强辐射下照射1小时（记为"Cells – light"，即光照组细胞）。CAC天线复合物通过蔗糖梯度离心法分离，选取第1组分用于后续分析（详见图5A）。采用甲醇萃取类胡萝卜素，随后通过高效液相色谱（High Performance Liquid Chromatography, HPLC）完成分离。值得注意的是，本数据为特定色素的峰面积相对于叶绿素a（chlorophyll a）的相对值。经高光胁迫处理后，总类胡萝卜素与叶绿素a的含量均未发生显著变化。