Single nuclei RNAseq of skin sensory neurons during psoriasis

The peripheral nervous system plays important roles in orchestrating tissue function in health and disease. In this work, we perform single nuclei RNAseq of skin sensory neuron DRGs in a mouse model of psoriasis to interrogate the impact of skin inflammation on the function and transcriptome of skin sensory neurons. Overall design: 10000 nuclei per lane isolated from dorsal root ganglion of mice (total of 6 lanes, 3 Control and 3 S. aureus IMQ) were loaded on a Chromium Single Cell Controller (10X Genomics) to encapsulate the nuclei into droplets. The gene expression (GEX) library was prepared using Chromium Single Cell 5' v2 reagent kits following the manufacturer's instructions, and sequenced on an Illumina Nextseq2000 platform

外周神经系统（peripheral nervous system）在调控健康与疾病状态下的组织功能中发挥关键作用。
本研究针对银屑病小鼠模型中的皮肤感觉神经元背根神经节（dorsal root ganglion，DRGs）开展单细胞核RNA测序（single nuclei RNAseq），以探究皮肤炎症对皮肤感觉神经元功能及转录组的影响。
实验设计概述：从小鼠背根神经节中分离得到的细胞核，每个测序泳道加载10000个，总共计6个泳道，其中对照组与金黄色葡萄球菌（S. aureus）咪喹莫特（IMQ）组各3个，将其置于Chromium单细胞控制器（10X Genomics）中以将细胞核包裹进微滴。按照制造商的操作说明，使用Chromium单细胞5'端v2试剂试剂盒制备基因表达（gene expression，GEX）文库，并在Illumina Nextseq2000测序平台上完成测序。