Common lymphoid progenitor derivation of plasmacytoid dendritic cells is mediated by Bcl11a Â

Plasmacytoid dendritic cells (pDC) specialize in type I interferon production to coordinate innate and adaptive immunity. We identify two pDC subsets in vivo, evaluate their genome-wide expression, and demonstrate a developmental requirement for Bcl11a to provide insight into this enigmatic cell type. Overall design: ChIP sequenicng was performed for a transcription factor of BCL11A in the Nalm6 B cell line and the Raji B cell line. Input was sequenced and used as a control.

浆细胞样树突状细胞（plasmacytoid dendritic cells, pDC）专司I型干扰素产生，以协调固有免疫与适应性免疫应答。本研究在体内鉴定出两类pDC亚群，对其全基因组表达谱进行了分析，并证实Bcl11a在其发育过程中的必需作用，以期为解析这类尚不明朗的细胞类型提供新的视角。实验设计概述：在Nalm6 B细胞系与Raji B细胞系中，针对转录因子BCL11A开展了染色质免疫共沉淀测序（ChIP sequencing）；同时对Input样本进行测序并作为对照。