Gene expression changes of o-anisidine hydrochloride-induced in renal cortex of rats

This study is to develop a short term and highly accurate prediction method of renal carcinogenicity based on gene expression profile of rats administrated by carcinogens. We conducted 28 days-repeated dose experiments in male SD rats with o-anisidine hydrochloride, and the gene expression profiles of renal cortex were analyzed using custom microarrays. Experimental design is the two group (control group vs. o-anisidine hydrochloride treated group). Three biological replicates were performed for each group.

本研究旨在基于致癌物染毒大鼠的基因表达谱，开发一种短期且高精度的肾脏致癌性预测方法。本研究以邻茴香胺盐酸盐（o-anisidine hydrochloride）对雄性SD大鼠开展为期28天的重复染毒实验，并采用定制基因芯片（custom microarrays）分析大鼠肾皮质的基因表达谱。实验设计分为两组：对照组与邻茴香胺盐酸盐染毒组。每组均设置3次生物学重复。