ChIP-Chip tiling array for H3k27ac in mouse MV+ (lens epithelium) and RAG cells ( renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

Enhancer identification using H3k27ac ChIP-ChIP in mouse MV+ and RAG, using custom tilling array covering a 66 Mb region around Pax6 (Chr2:75,000,000-141,000,000) mm9. Additional loci and regions, Sox2 (chr3:32,000,000-37,000,000), Otx2 (chr14:48,500,000-50,500,000), Rax (chr18:65,500,000-68,000,000), Hba a-globin (chr11:32,050,000-32,450,000), Six3 (chr17:85,200,000-86,700,000), Sox1 (chr8:11,800,000-12,800,000), Mab21L2 (chr3:85,850,000-86,850,000), Bmp4 (chr14:46,500,000-47,500,000), NeuroD1 (chr2:79,050,000-79,600,000), Prox1 (chr1:191,700,000-192,700,000), Hmx1 (chr5:34,700,000-36,200,000), H19-Igf2 (chr7:149500000-150500000), Sox9 (chr11:110000000-116000000). Two cell lines, with two biological replicates of MV+ and RAG H3K27ac ChIP hybrisdised verus input DNA.

本数据集旨在通过实验鉴定增强子，采用针对小鼠MV+与RAG细胞系的H3K27ac 染色质免疫沉淀-芯片（ChIP-ChIP）技术，使用定制平铺阵列芯片覆盖Pax6基因周边66 Mb的基因组区域（对应小鼠mm9参考基因组2号染色体：75,000,000-141,000,000）。此外还涵盖以下额外基因座与基因组区域：Sox2（3号染色体：32,000,000-37,000,000）、Otx2（14号染色体：48,500,000-50,500,000）、Rax（18号染色体：65,500,000-68,000,000）、Hba α-珠蛋白（11号染色体：32,050,000-32,450,000）、Six3（17号染色体：85,200,000-86,700,000）、Sox1（8号染色体：11,800,000-12,800,000）、Mab21L2（3号染色体：85,850,000-86,850,000）、Bmp4（14号染色体：46,500,000-47,500,000）、NeuroD1（2号染色体：79,050,000-79,600,000）、Prox1（1号染色体：191,700,000-192,700,000）、Hmx1（5号染色体：34,700,000-36,200,000）、H19-Igf2（7号染色体：149,500,000-150,500,000）、Sox9（11号染色体：110,000,000-116,000,000）。本数据集包含2种细胞系，分别对MV+与RAG细胞的H3K27ac ChIP样本设置2次生物学重复，并将其与输入对照DNA进行杂交检测。