Changes in the gene expression in the inferior colliculus of the sound stimulated rats both Wistar and the strain susceptible to audiogenic seizures WAR

Genetic animal models of epilepsy are an important tool for further understanding the basic cellular mechanisms underlying epileptogenesis and for developing novel antiepileptic drugs. We conducted a comparative study of gene expression in the inferior colliculus, a nucleus that triggers audiogenic seizures, using two animal models, the Wistar audiogenic rat (WAR) and the genetic audiogenic seizure hamster (GASH:Sal). For this purpose, both models were subjected to auditory stimulation, and 60 minutes after stimulation, the inferior colliculi were collected. As a control, intact Wistar rats and Syrian hamsters were subjected to identical stimulation and tissue preparation protocols to those performed on the experimental animals.癫痫遗传动物模型是深入解析癫痫发生（epileptogenesis）背后核心细胞机制、研发新型抗癫痫药物的重要工具。本研究以听源性发作（audiogenic seizures）易感Wistar大鼠（Wistar audiogenic rat, WAR）与遗传型听源性发作仓鼠（GASH:Sal）为研究对象，对其触发听源性发作的下丘（inferior colliculus）开展基因表达对比分析。为此，对两类模型动物施加听觉刺激，并于刺激结束后60分钟采集其下丘组织。设置正常Wistar大鼠与叙利亚仓鼠作为对照组，对其施加与实验组完全一致的听觉刺激及组织制备流程。