Next generation sequencing of the transcriptome of NPC cell line C666_1 under various drug treatments.. Next generation sequencing of the transcriptome of NPC cell line C666_1 under various drug treatments.

The NPC cell line C666_1 is known to be infected with Epstein Barr virus (EBV), and to have high expression of SSTR2A. The cell line was treated with PENN221 (an SSTR2A targeted drug conjugate) , Belinostat (a histone deacetylase inhibitor), DMSO (negative control) or left untreated (negative control). Both PENN221 and Belinstat resulted in increased cell death of the cell line. Treated samples were sequenced at 24h and 72h post treatment. Overall design: mRNA profiles of untreated and PEN221, Belinostat or DMSO treated NPC cell line C666_1 at 24h and 72h post treatment.

已知NPC细胞系（NPC cell line）C666-1感染了EB病毒（Epstein Barr virus, EBV），且高表达生长抑素受体2A（SSTR2A）。该细胞系分别经靶向SSTR2A的药物偶联物PENN221、组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor）贝利司他（Belinostat）、二甲基亚砜（DMSO，阴性对照）处理，或不做任何处理（亦为阴性对照）。PENN221与贝利司他均可导致该细胞系的细胞死亡水平升高。处理后的样本分别在处理后24小时与72小时进行测序。整体实验设计：未处理及经PENN221、贝利司他、二甲基亚砜处理的NPC细胞系C666-1在处理后24小时与72小时的mRNA表达谱。