Expression data from liver of obese (with or without type 2 diabetes) and lean human subjects.

Hepatic lipid accumulation is an important complication of obesity linked to risk for type 2 diabetes. To identify novel transcriptional changes in human liver which could contribute to hepatic lipid accumulation and associated insulin resistance and type 2 diabetes (DM2), we evaluated gene expression and gene set enrichment in surgical liver biopsies from 13 obese (9 with DM2) and 5 control subjects, obtained in the fasting state at the time of elective abdominal surgery for obesity or cholecystectomy. RNA was isolated for cRNA preparation and hybridized to Affymetrix U133A microarrays. Human liver samples were obtained from 5 lean control subjects undergoing elective cholecystectomy and 13 obese subjects (with or without Type 2 diabetes) undergoing gastric bypass surgery. Subjects with diabetes were classified as either well-controlled or poorly-controlled.

肝脏脂质蓄积是肥胖的重要并发症，与2型糖尿病的发病风险密切相关。为鉴定人类肝脏中可能参与肝脏脂质蓄积及相关胰岛素抵抗、2型糖尿病（DM2）发生的新型转录变化，我们对13名肥胖受试者（其中9名合并2型糖尿病）及5名对照受试者的手术肝脏活检标本开展了基因表达与基因集富集分析，所有标本均采集于择期肥胖手术或胆囊切除术时的空腹状态下。我们提取RNA以制备互补RNA（cRNA），并将其与Affymetrix U133A基因芯片进行杂交。本研究的人类肝脏样本还取自5名接受择期胆囊切除术的消瘦对照受试者，以及13名接受胃旁路手术的肥胖受试者（合并或不合并2型糖尿病）。其中合并糖尿病的受试者被分为控制良好组与控制不佳组。