H3K27me3 (Abcam lot3).Kc.Affy 2

modENCODE_submission_5136 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: Kc167; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Genotype: se/e; Sex: Female; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line Kc167; Antibody H3K27me3 (Abcam lot3) (target is H3K27me3)

modENCODE_submission_5136 本次提交源自Gary Karpen的modENCODE项目。如需查阅modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在明确黑腹果蝇（Drosophila melanogaster）基因组中主要组蛋白修饰的分布位点。所研究的修饰参与DNA复制、基因表达、基因沉默及遗传等核心染色体功能。本研究将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺芯片的技术方案。初步将利用3种细胞系与2个胚胎阶段的染色质开展定位分析，后续还会将部分靶蛋白的分析拓展至另外4种动物组织/发育阶段。
关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：ChIP-chip。
生物样本来源：细胞系：Kc167；组织类型：胚胎来源细胞系；发育阶段：晚期胚胎阶段；基因型：se/e；性别：雌性；重复次数：4；实验因子：Kc167细胞系；抗体：H3K27me3（Abcam 批次3）（靶标为H3K27me3）