Longitudinal analysis of pancreatic adenocarcinoma development reveals transient gene expression signatures (I. 10-22 cell RNA-Seq)

We performed a transcriptome time-course analysis of genetically tagged 10-22 cells through the progression from PanINs to PDAC in mice. The results were validated using the Cancer Genome Atlas (TCGA) PDAC dataset, human clinical PanIN/PDAC tissues, and well-established murine PDAC model. Overall design: Each 10-22 cell tagged with KiR-EG was subcutaneously transplanted into 4-6 weeks old female NOD-SCID-IL2Rgc null (NSG) mice and harvested 1 week, 2 weeks, 3 months, 6 months, and 9 months. 10-22 KIR-EG clone 6 that was cultured in pluripotent ES culture condition were harvested as day 0 control.

本研究对经遗传标记的10-22细胞在小鼠体内从胰腺上皮内瘤变（Pancreatic Intraepithelial Neoplasia, PanINs）进展至胰腺导管腺癌（Pancreatic Ductal Adenocarcinoma, PDAC）的过程开展了转录组时间序列分析。研究结果通过癌症基因组图谱（Cancer Genome Atlas, TCGA）PDAC数据集、人类临床PanIN/PDAC组织以及成熟的小鼠PDAC模型得到了验证。整体实验设计：每株经KiR-EG标记的10-22细胞均被皮下移植至4-6周龄的雌性NOD-SCID-IL2Rgc缺陷型（NSG）小鼠体内，并分别于移植后1周、2周、3个月、6个月及9个月时取材；将在多能胚胎干细胞培养条件下培养的10-22 KIR-EG克隆6作为第0天对照组取材。