Replication Data for: Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines

This dataset is about analyses of two Merkel cell polyomavirus (MCPyV)-negative and two MCPyV–positive Merkel cell lines secretomic proteins.
Abstract: Extracellular vesicles or exosomes constitute an evolutionarily conserved mechanism of intercellular signaling. Exosomes are gaining an increasing amount of attention due to their role in pathologies, including malignancy, their importance as prognostic and diagnostic markers, and their potential as a therapeutic tool. Merkel cell carcinoma (MCC) is an aggressive form of skin cancer with a poor prognosis. Because an effective systemic treatment for this cancer type is currently not available, an exosome-based therapy was proposed. However, comprehensive secretome profiling has not been performed for MCC. To help unveil the putative contribution of exosomes in MCC, we studied the protein content of MCC-derived exosomes. Since approximately 80% of all MCC cases contain Merkel cell polyomavirus (MCPyV), the secretomes of two MCPyV-negative and two MCPyV-positive MCC cell lines were compared. We identified with high confidence 164 exosome-derived proteins common for all four cell lines that were annotated in ExoCarta and Vesiclepedia databases. These include proteins implicated in motility, metastasis and tumor progression, such as integrins and tetraspanins, intracellular signaling molecules, chaperones, proteasomal proteins, and translation factors. Additional virus-negative and virus-positiveMCC cell lines should be examined to identify highly representative exosomal proteins that may provide reliable prognostic and diagnostic biomarkers, as well as targets for treatment in the future. Data are available via ProteomeXchange with identifier PXD004198.

本数据集针对两种默克尔细胞多瘤病毒（Merkel cell polyomavirus）阴性及两种MCPyV阳性的默克尔细胞系分泌组蛋白展开分析。
摘要：细胞外囊泡或外泌体是一种进化上保守的细胞间信号传导机制。外泌体因其在包括恶性肿瘤在内的病理过程中的作用、作为预后与诊断标志物的重要性，以及作为治疗工具的潜力而日益受到关注。默克尔细胞癌（Merkel cell carcinoma）是一种侵袭性皮肤癌，预后较差。由于目前尚无针对该类型癌症的有效系统治疗方法，因此提出了基于外泌体的治疗方案。然而，针对默克尔细胞癌的全面分泌组谱分析尚未开展。为揭示外泌体在默克尔细胞癌中的潜在作用，我们研究了默克尔细胞癌来源外泌体的蛋白质组成。鉴于约80%的默克尔细胞癌病例均含有默克尔细胞多瘤病毒（Merkel cell polyomavirus, MCPyV），我们对比了两种MCPyV阴性与两种MCPyV阳性默克尔细胞系的分泌组。我们通过高置信度鉴定出164种所有四种细胞系共有的外泌体衍生蛋白，这些蛋白已在ExoCarta和Vesiclepedia数据库中注释。其中包括与细胞运动、转移及肿瘤进展相关的蛋白（如整合素和四跨膜蛋白）、细胞内信号分子、分子伴侣、蛋白酶体蛋白及翻译因子。未来需进一步研究更多病毒阴性及病毒阳性的默克尔细胞系，以鉴定具有高度代表性的外泌体蛋白，这些蛋白或可作为可靠的预后与诊断生物标志物，以及未来的治疗靶点。数据可通过ProteomeXchange获取，标识符为PXD004198。