RNA-seq data from wildtype, STAT5A-knockout and STAT5B-knockout BCR/ABLp185+ transformed mouse bone marrow cell lines

RNA-Seq of immortalized Stat5a-/-, Stat5b-/-, and wildtype BCR/ABLp185+ cell lines was used to identify STAT5A and STAT5B specific target genes. Overall design: mRNA from immortalized Stat5a-/-, Stat5b-/-, and wildtype BCR/ABLp185+ cell lines (3 technical replicates) were prepared using the Lexogen SENSE mRNA-Seq library preparation kit. Single-end, 50bp sequencing was performed on an Illumina HiSeq-2500 sequencer. Reads were mapped to the GENECODE M13 genome.

本研究采用永生化的纯合Stat5a基因敲除（Stat5a-/-）、纯合Stat5b基因敲除（Stat5b-/-）及野生型BCR/ABLp185阳性细胞系的RNA-Seq数据，以鉴定STAT5A与STAT5B的特异性靶基因。
实验整体设计：提取上述三类永生化细胞系的信使RNA（mRNA）（各设置3次技术重复），使用Lexogen SENSE mRNA-Seq文库制备试剂盒完成文库构建；随后在Illumina HiSeq-2500测序仪上进行单端50bp测序；将测序获得的测序读段（reads）比对至GENCODE M13参考基因组。