Genome-wide profiling of gene expression and transcription factors binding reveals new insights into the mechanisms of gene regulation during Drosophila spermatogenesis [ChIP-Seq]. Drosophila melanogaster

To investigate the mechanisms of gene regulation during Drosophila spermatogenesis, we studied the effects of comr and can mutations on the chromatin of the cells in Drosophila testes by H3K27me3 ChIP-Seq. Overall design: ChIP-Seq of H3K27me3 histone modification in comr- and can-mutant testes of Drosophila melanogaster. We processed two biological replicates of every sample and corresponding input specimen.

为探究果蝇精子发生过程中的基因调控机制，我们采用H3K27me3染色质免疫共沉淀测序（ChIP-Seq）技术，分析了comr与can基因突变对黑腹果蝇睾丸细胞染色质的影响。实验整体设计：针对黑腹果蝇comr突变型、can突变型睾丸，开展H3K27me3组蛋白修饰的ChIP-Seq检测。本研究对每个样本及其对应的输入对照样本均设置了两次生物学重复。