Histone tail modification profiles of human renal tubule epithelial cells. Homo sapiens

To annotate the regulatory elements in the renal tubule epithelial cells, we profiled 6 histone ChIP-seq in the human kidney epithelical cells (HKC8). Overall design: We pulled down the DNA with specific antibodies of interests against histone tail modifications in the human rebal tubule epithelial cells. The result can be interpretated with ChromHMM for different states.

为注释肾小管上皮细胞中的调控元件，我们针对人肾小管上皮细胞系HKC8开展了6种组蛋白染色质免疫共沉淀测序（ChIP-seq）。
整体实验设计：我们使用针对组蛋白尾部修饰的特异性目标抗体，从该细胞中免疫沉淀富集靶DNA片段。该测序结果可结合ChromHMM工具进行分析，以解析不同的染色质状态。