Chromatin integration labeling for mapping multiple DNA binding proteins and modifications with low input.. Chromatin integration labeling for mapping multiple DNA binding proteins and modifications with low input.

We established a novel epigenomic profiling method multi–target ChIL–seq, mtChIL-seq, which enables the detection of the DNA binding proteins and histone modifications simultaneously using a same sample. Overall design: We performed mtChIL-seq on C2C12 cells with various cell numbers (100, 1k and 10k cells) against various histone modifications and DNA binding proteins.

本研究建立了一种新型表观基因组谱分析方法——多靶点ChIL-seq（multi-target ChIL-seq，mtChIL-seq），该方法可利用同一样本同时检测DNA结合蛋白与组蛋白修饰。整体实验设计：我们针对多种组蛋白修饰及DNA结合蛋白，在细胞数量分别为100个、1000个和10000个的C2C12细胞中开展了mtChIL-seq实验。