4hpi - 6wpi Gene Expression in HCMV-Infected Primary Human Monocytes

To investigate how HCMV alters monocyte gene expression over time, we collected RNA from HCMV-infected primary human monocytes (3 donors) from 4 hours to 6 weeks post infection and analyzed the differential gene expression by RNAseq. Overall design: Primary human monocytes were mock-infected or HCMV-infected. RNA was collected at 4 hpi, 24 hpi, 2 wpi, 4 wpi, and 6 wpi. 3 replicates are included.

为探究人类巨细胞病毒（Human Cytomegalovirus, HCMV）随时间对单核细胞基因表达的调控模式，我们从3名供体获取原代人单核细胞，对其进行人类巨细胞病毒（HCMV）感染，并在感染后4小时至6周的多个时间点采集总RNA，通过RNA测序（RNAseq）技术分析差异基因表达情况。实验整体设计：将原代人单核细胞分为模拟感染组与HCMV感染组，分别在感染后4小时（4 hpi）、24小时（24 hpi）、2周（2 wpi）、4周（4 wpi）及6周（6 wpi）五个时间点采集RNA样本，每组设置3次生物学重复。