TABLE 1 in Microfungi associated with dead caterpillars and spiders in Xishuangbanna Tropical Botanical Garden, P. R. China

TABLE 1. The PCR conditions and primers used in this study.
LocusDefinitionPrimerPrimer DNA sequence (5'-3')Annealing (PCR)Referencesgapdhglyceraldehyde 3-phosphate dehydrogenasegpd1CAACGGCTTCGGTCGCATTGgpd2GCCAAGCAGTTGGTTGTGCa60 °C, 30 s bBerbee et al. (1999)ITSinternal transcribed spacerITS4TCCGTAGGTGAACCTGCGGITS5TCCTCCGCTTATTGATATGCa55 °C, 50 sbWhite et al. (1990)LSUnuclear ribosomal large subunitLR0RGTACCCGCTGAACTTAAGCLR5ATCCTGAGGGAAACTTCa55 °C, 50 sbVilgalys & Hester (1990)rpb1DNA-dependent RNA polymerase II largest subunitAfGARTGYCCDGGD CAYTTYGGCrCGCATTCTTTCCATTAa49 °C, 1 minbLu et al. (2013)SSU18S ribosomal RNANS1GTAGTCATATGCTTGTCTCNS4CTTCCGTCAATTCCTTTAAGa55 °C, 50 sbWhite et al. (1990)tef1-αtranslation elongation factor 1 alpha983FGCYCCYGGHCAYCGTGAYTTYAT2218RATGACACCRACRGCRACRGTYTGa56 °C, 50 s bRehner & Buckley (2005)

表1 本研究使用的聚合酶链式反应（PCR）条件与引物
基因座 功能定义 引物 引物DNA序列（5'→3'） 退火（PCR）条件 参考文献
gapdh 甘油醛-3-磷酸脱氢酶（glyceraldehyde 3-phosphate dehydrogenase） gpd1 CAACGGCTTCGGTCGCATTG gpd2 GCCAAGCAGTTGGTTGTGC a60℃，30s bBerbee等(1999)
ITS 内转录间隔区（internal transcribed spacer） ITS4 TCCGTAGGTGAACCTGCGG ITS5 TCCTCCGCTTATTGATATGC a55℃，50s bWhite等(1990)
LSU 核核糖体大亚基（nuclear ribosomal large subunit） LR0R GTACCCGCTGAACTTAAGC LR5 ATCCTGAGGGAAACTTC a55℃，50s bVilgalys & Hester(1990)
rpb1 DNA依赖的RNA聚合酶II最大亚基（DNA-dependent RNA polymerase II largest subunit） AfGARTGYCCDGGD CAYTTYGGCr CGCATTCTTTCCATTA a49℃，1min bLu等(2013)
SSU 18S核糖体RNA（18S ribosomal RNA） NS1 GTAGTCATATGCTTGTCTC NS4 CTTCCGTCAATTCCTTTAAG a55℃，50s bWhite等(1990)
tef1-α 翻译延伸因子1α（translation elongation factor 1 alpha） 983F GCYCCYGGHCAYCGTGAYTTYAT 2218R ATGACACCRACRGCRACRGTYT a56℃，50s bRehner & Buckley(2005)