Towards 3D structure determination and dynamics of biological and non-biological samples using TR-SSX methods

This BAG proposal aims to conduct room temperature time-resolved serial crystallography experiments at the new ID29 beamline at ESRF. We intend to collect
full data sets on microcrystals of two medically relevant proteins, the penicillin binding protein 2a (PBP2a) of methicillin resistant staphyloccocus aureus (MRSA)
and the human the NAP(P)H:quinone oxidoreductase 1 (NQO1) protein, with their respective ligands. As well, we aim at conducting pump-probe time-resolved
crystallography experiments on microcrystals of metal organic frameworks (MOFs). The good quality of all crystalline samples has been proved either at ESRF
beamlines ID30-A1 and ID30-A3, or at other synchrotron beamlines. At ID29, they offer a variety of sample delivery devices including high viscosity injectors, tape
drives, fixed target supports, and microfluidic devices, that will be employed in our experiments depending on sample needs.

本BAG提案旨在欧洲同步辐射装置（ESRF）新建的ID29光束线开展室温时间分辨串行晶体学实验。我们计划收集
两种具有医学相关性的蛋白质微晶的完整数据集：耐甲氧西林金黄色葡萄球菌（MRSA）的青霉素结合蛋白2a（PBP2a）
以及人源NAP(P)H：醌氧化还原酶1（NQO1）蛋白，均包含其各自的配体。此外，我们拟对金属有机框架（MOFs）微晶
开展泵浦-探测时间分辨晶体学实验。所有晶体样品的优良质量已在ESRF光束线ID30-A1、ID30-A3或其他同步辐射
光束线得到验证。ID29光束线提供多种样品递送装置，包括高粘度注射器、胶带驱动装置、固定靶支架和微流控装置，
我们将根据样品需求选用这些装置开展实验。