'A CRISPR-Cas9 mutation in sox9b long intergenic noncoding RNA (slincR) affects zebrafish development, behavior, and regeneration
收藏NIAID Data Ecosystem2026-05-02 收录
下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP417750
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资源简介:
transcriptomic data for 48 hpf Wildtype, slincRosu3 (sg6; slincR +18 bp mutant) and BigD (slincR -131 bp mutant) zebrafish embryos developmentally exposed to TCDD. Overall design: Two treatments- Controls ("0") and 1 ng/mL TCDD ("1") -exposed from 6-48 h post fertilization (hpf). Embryos collected at 48 hpf and then run through library prep and sequencing on a Beijing Genomics Institute DNBseq-500 platform, with 100 bl PE
本数据集为发育阶段暴露于2,3,7,8-四氯二苯并对二噁英(TCDD)的野生型、slincRosu3(sg6;slincR +18 bp突变体)及BigD(slincR -131 bp突变体)斑马鱼胚胎的转录组数据,所有样本采集自受精后48小时(hours post fertilization,hpf)。
实验设计概述:设置两组处理——对照组(标记为"0")与1 ng/mL TCDD处理组(标记为"1"),暴露时长为受精后6至48小时。于受精后48小时收集胚胎,随后完成文库制备与测序,测序平台为北京基因组研究所(Beijing Genomics Institute)DNBseq-500平台,采用100 bp双端测序模式。
创建时间:
2024-07-11



