Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.

摘要 本研究评估了编码雌激素受体1（ESR1）、雌激素受体2（ESR2）与维生素D受体（VDR）的基因多态性，以及可结合ESR1与VDR的微小RNA17（microRNA17）的多态性，与牙髓治疗后持续性根尖周炎（persistent apical periodontitis, PAP）之间的关联。本研究纳入162名至少1年前完成牙髓治疗，且根管治疗初始阶段即存在根尖周炎的患者。通过临床与影像学检查评估患者是否存在持续性根尖周炎或健康的根尖周组织（即愈合状态）。采集唾液样本以提取基因组DNA。采用实时PCR（real-time PCR）对ESR1（rs2234693与rs9340799）、ESR2（rs1256049与rs4986938）、VDR（rs739837与rs2228570）及微小RNA17（microRNA17）进行基因分型。采用卡方检验分析基因型与等位基因频率的分布，并开展单倍型分析。本研究中，89名患者被归入"愈合组"，73名患者被归入"PAP组"。研究未发现所分析的等位基因与基因型多态性与持续性根尖周炎存在关联（p>0.05）。单倍型分析同样未显示存在关联（p>0.05）。综上，ESR1、ESR2、VDR及微小RNA17的基因多态性与持续性根尖周炎并无关联。