Single cell Transcriptome characterization of the effects of exosomes from different sources on testicular tissue of male mice

Collect the blood circulation exosomes from antibiotic treated or wild type mice, and then inject them into the recipient mice through tail vein, collect the testicular tissue of the recipient mice, conduct transcriptome sequencing, and analyze and compare gene expression differences Inject PBS (BP), blood exosomes from wild-type mice (BE), and intestinal microbiota imbalance mice (BdE) into the mouse model of azoospermia induced by Baixiaoan.

收集经抗生素处理或野生型小鼠体内的血液循环外泌体（exosomes），通过尾静脉注射至受体小鼠体内；随后采集受体小鼠的睾丸组织进行转录组测序（transcriptome sequencing），并分析比较基因表达差异。此外，将PBS（BP）、野生型小鼠血液外泌体（BE）以及肠道菌群失调小鼠的血液外泌体（BdE）注射至白消安（Baixiaoan）诱导的无精子症小鼠模型中。