Spatial transcriptomic reveals the cellular landscape of bladder cancer

The cellular diversity of stromal compartment within the tumor microenvironment might change dynamically as tumors evolve that allowing distant dissemination of tumor cells and informing the treatment options. Importantly, the localization of cells in tumor microenvironment indicates the biological function of cells. Thus, we used spatial transcriptomic to reveal the cellular landscape and corresponding cellular localization in bladder cancer. Overall design: The xx-case paraffin sections of bladder cancer were obtained from Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University. Then, the tissue sections were subjected to the Visium Spatial Tissue Optimization Slide kit to fit the time for permeabilization by generating fluorescently labeled cDNA tissue prints. Subsequently, the permeabilization processes were conducted and the spatially barcoded, full-length cDNA is amplified via PCR to generate sufficient mass for library construction. The library sequencing was performed on the Illumina novaseq 6000.

肿瘤微环境（tumor microenvironment）内间质室的细胞多样性可随肿瘤进展发生动态变化，这一变化不仅会促进肿瘤细胞的远处播散，还可为治疗方案的选择提供参考。值得注意的是，肿瘤微环境中细胞的定位可反映其生物学功能。因此，本研究利用空间转录组学（spatial transcriptomic）技术，解析膀胱癌中的细胞图谱及其对应的细胞定位。整体实验设计：本研究从中山大学孙逸仙纪念医院获取了xx例膀胱癌石蜡切片组织。随后，将组织切片置于Visium空间组织优化玻片试剂盒（Visium Spatial Tissue Optimization Slide kit）中，通过生成荧光标记的cDNA组织印渍来确定最优透化时长。后续开展透化处理，将空间条形码标记的全长cDNA通过PCR扩增以获得文库构建所需的足够物料。最终在Illumina NovaSeq 6000测序平台上完成文库测序。