Genome-wide characterization of fragile and resistant nucleosomes in response to cold stress in maize [MH-seq]

Distinct nucleosome architectures decode key regulatory information related to various cellular processes. However, how fragile (Fra-Nus) and resistant (Res-Nus) nucleosomes, with sensitivity and resistance to MNase cleavage, respectively, are reprogrammed and their potential biological implications under cold treatment is completely uncharacterized in plants. To this end, we conducted MNase-seq with light, medium and heavy MNase digestion in combination with multi-omics data (RNA-seq, ChIP-seq, MH-seq and resequencing data) between COLD and CK. Overall design: MNase-seq with light, medium and heavy MNase-seq between COLD and CK. Chip-seq of H3K27me3, H3k4me3, H3k9ac between COLD and CK. RNA-seq between COLD and CK.MH-seq between COLD and CK.

独特的核小体架构可解码与多种细胞过程相关的关键调控信息。然而，分别对微球菌核酸酶（MNase）切割具有敏感性与抗性的脆弱型核小体（Fra-Nus）与抗性型核小体（Res-Nus），其重编程过程以及低温处理下的潜在生物学意义在植物中尚未得到任何表征。为此，我们在低温处理组（COLD）与对照组（CK）之间，采用轻、中、重度微球菌核酸酶消化开展MNase测序（MNase-seq），并结合多组学数据（RNA测序[RNA-seq]、染色质免疫共沉淀测序[ChIP-seq]、MH-seq以及重测序数据）进行研究。整体实验设计如下：在COLD与CK组间开展轻、中、重度酶切的MNase-seq；在COLD与CK组间开展针对H3K27me3、H3K4me3、H3K9ac的ChIP-seq；在COLD与CK组间开展RNA-seq；在COLD与CK组间开展MH-seq。