Dm_DmD20-c2_TotalRNA_p200_182-184-186_38bp

modENCODE_submission_56 This submission comes from a modENCODE project of Susan Celniker. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will generate over 600 RNA samples in biological triplicate and use them to generate expression profile maps detailing the sites of transcription across the fly genome using whole genome tiling arrays at 38 bp resolution as a broad survey of the transcriptome and 7 bp arrays resolution to identify at high resolution transcripts ends, splice sites, and small RNAs. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis BIOLOGICAL SOURCE: Cell Line: ML-DmD20-c2 TISSUE: antenna disc-derived cell-line GENOTYPE: y v f mal SEX: Male NUMBER OF REPLICATES: 3; Replicate 1 applied to 1 array(s), Replicate 2 applied to 1 array(s), Replicate 3 applied to 1 array(s), No dye swap. EXPERIMENTAL FACTORS: Cell Line ML-DmD20-c2

modENCODE_submission_56
本提交数据源自Susan Celniker主导的模式生物DNA元件百科全书（modENCODE）项目。若需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。

项目目标：我们将制备600余份生物学三重重复的RNA样本，以此构建基因表达谱图谱，详细呈现果蝇基因组全域的转录位点分布；实验采用38碱基对（bp）分辨率的全基因组平铺芯片（whole genome tiling array）对转录组进行广谱筛查，同时辅以7碱基对分辨率的芯片，以高精准度鉴定转录末端、剪接位点及小分子RNA。

有关数据使用的条款与细则，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

关键词：转录组平铺芯片分析（Transcript tiling array analysis）
实验类型：转录组平铺芯片分析（Transcript tiling array analysis）
生物来源：细胞系：ML-DmD20-c2
组织：触角盘衍生细胞系
基因型：y v f mal
性别：雄性
重复次数：3次生物学重复；重复1对应1张芯片，重复2对应1张芯片，重复3对应1张芯片，未进行染料互换。
实验因素：细胞系ML-DmD20-c2