RNAseq analysis of CC strains after influenza A virus infection identifies genetic control of the host response. RNAseq analysis of CC strains after influenza A virus infection identifies genetic control of the host response

Gene expression changes in the lung was studied by RNAseq in different CC mouse strains infected with influenza A virus. Results: Strong differences between the genetically CC mouse strains can be observed in the activation of host defense genes. These differences can be related to genetic variants in the genome of the CC strains. Furthermore, different strains exhibit variant susceptibility to infections (body weight loss and survival). These differences can be correlated to differences in gene expression profiles. Overall design: Female mice from 11 CC mouse strains were infected with mouse-adapted H3N2 virus and lungs were harvested at day3, 5 nd 8 after infection. Total RNA was isolated and mRNA was sequenced.

本研究采用RNA测序（RNAseq）技术，对感染甲型流感病毒（influenza A virus）的不同CC小鼠品系（CC mouse strains）的肺部基因表达变化展开研究。
结果：不同遗传背景的CC小鼠品系在宿主防御基因的激活水平上呈现显著差异，该差异与CC品系基因组内的遗传变异存在关联。此外，不同品系对病毒感染表现出各异的易感性（以体重减轻与存活率为衡量指标），此类易感性差异与基因表达谱的差异具有相关性。
实验整体设计：选取11个CC小鼠品系的雌性小鼠，使用小鼠适应性H3N2病毒（mouse-adapted H3N2 virus）进行感染，并分别于感染后第3、5、8天采集肺部组织。提取总RNA后对mRNA进行测序。