Hearing in C57BL/6J mice treated with sildenafil citrate.

C57BL/6J mice at 45 days after birth were treated with vehicle (N = 9) or sildenafil (N = 7). Hearing threshold value in each cell in column 3 represents average ABR threshold derived from 9 mice (N = 9) at that time point. ABR thresholds were recorded from the same group of mice over time, day 0, 7, 21 and 30 (also referred to as ‘longitudinal’ testing), to assess cumulative effects of sildenafil treatment on hearing. It should be noted that ‘day 0’ is pre-treatment or baseline ABR. The same explanation holds for column 4 (treated mice, N = 7). *A repeated measure ANOVA, non parametric, using a Friedman test was used to compare the hearing thresholds of the mice over time. A P value less than 0.05 is considered significant.

出生后45天的C57BL/6J小鼠被分为两组，分别接受赋形剂处理（N=9）与西地那非处理（N=7）。第三列各单元格中的听阈数值，均为对应时间点下9只小鼠（N=9）的平均听性脑干反应（ABR）阈值。为评估西地那非治疗对听力的累积效应，本研究对每一处理组的同批小鼠在第0、7、21、30天进行了ABR阈值检测（亦称为"纵向"检测）。需说明的是，“第0天”为治疗前的基线ABR检测结果。第四列（给药处理小鼠，N=7）的说明与此一致。*采用非参数重复测量方差分析——弗里德曼（Friedman）检验，对不同时间点的小鼠听阈进行比较。当P值小于0.05时，判定差异具有统计学意义。