Gene expression in laboratory models and primary tumors in Small Cell Lung Cancer. Homo sapiens

Gene expression was measured on the Affymetrix platform in primary xenografts, xenograft-derived cell lines, secondary xenografts, normal lung, and primary tumors obtained from chemotherapy naive Small Cell Lung Cancer (SCLC). The SCLC primary xenografts were serially propagated in vivo in immunodeficient mice. Cell lines were derived from each xenograft and grown for 6 months using conventional tissue culture conditions. Secondary xenografts were obtained from cell cultures by re-implantation in immunodeficient mice. Such SCLC laboratory models were analyzed along with conventional SCLC cell lines and the derivative secondary xenografts, with normal lung and primary tumors, to assess irreversible gene expression changes induced by culturing conditions. Overall design: SCLC primary xenografts were compared to the corresponding xenograft-derived cell lines, and to the secondary xenografts established from the cell lines using the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Gene expression from SCLC primary tumors was measured using the Affymetrix GeneChip Human Genome U133A 2.0 Array. 3 datasets: GSM380476-GSM380512, GSM380513-GSM380516, and GSM380517-GSM380520

本数据集通过Affymetrix平台检测了多类样本的基因表达水平，样本包括初治小细胞肺癌（Small Cell Lung Cancer, SCLC）患者的原代异种移植瘤、源自异种移植瘤的细胞系、继代异种移植瘤、正常肺组织以及原发肿瘤。其中，SCLC原代异种移植瘤在免疫缺陷小鼠体内进行了连续体内传代培养。从每一株异种移植瘤中分离得到细胞系，并采用常规组织培养条件培养长达6个月。继代异种移植瘤则通过将上述细胞系重新接种至免疫缺陷小鼠体内获得。为评估培养条件所诱导的不可逆基因表达变化，本研究对上述SCLC实验模型、常规SCLC细胞系、衍生的继代异种移植瘤、正常肺组织及原发肿瘤开展了联合分析。
实验整体设计如下：采用Affymetrix GeneChip Human Genome U133 Plus 2.0 基因芯片，将SCLC原代异种移植瘤分别与其对应的异种移植来源细胞系、以及由该细胞系构建的继代异种移植瘤进行表达谱比较。SCLC原发肿瘤的基因表达谱则通过Affymetrix GeneChip Human Genome U133A 2.0 基因芯片进行检测。本数据集包含3个独立子数据集，分别为GSM380476-GSM380512、GSM380513-GSM380516及GSM380517-GSM380520