Table_1_Identification of marker genes for spinal cord injury.DOCX

IntroductionSpinal cord injury (SCI) is a profoundly disabling and devastating neurological condition, significantly impacting patients’ quality of life. It imposes unbearable psychological and economic pressure on both patients and their families, as well as placing a heavy burden on society.
MethodsIn this study, we integrated datasets GSE5296 and GSE47681 as training groups, analyzed gene variances between sham group and SCI group mice, and conducted Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis based on the differentially expressed genes. Subsequently, we performed Weighted Gene Correlation Network Analysis (WGCNA) and Lasso regression analyses.
ResultsWe identified four characteristic disease genes: Icam1, Ch25h, Plaur and Tm4sf1. We examined the relationship between SCI and immune cells, and validated the expression of the identified disease-related genes in SCI rats using PCR and immunohistochemistry experiments.
DiscussionIn conclusion, we have identified and verified four genes related to SCI: Icam1, Ch25h, Plaur and Tm4sf1, which could offer insights for SCI treatment.

引言：脊髓损伤（Spinal cord injury, SCI）是一类严重致残且极具破坏性的神经系统疾病，可显著降低患者的生活质量。其不仅给患者及其家属带来沉重的心理与经济负担，同时也给社会造成了沉重的压力。
方法：本研究整合GSE5296与GSE47681数据集作为训练组，分析假手术组与脊髓损伤组小鼠的基因表达差异，并基于差异表达基因开展基因本体（Gene Ontology, GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析。随后，本研究进行了加权基因共表达网络分析（Weighted Gene Correlation Network Analysis, WGCNA）与Lasso回归分析。
结果：本研究筛选出4个特征性疾病相关基因：Icam1、Ch25h、Plaur及Tm4sf1。我们探究了脊髓损伤与免疫细胞之间的关联，并通过聚合酶链式反应（Polymerase Chain Reaction, PCR）与免疫组织化学实验，验证了上述疾病相关基因在脊髓损伤大鼠体内的表达情况。
讨论：综上，本研究筛选并验证了4个与脊髓损伤相关的基因：Icam1、Ch25h、Plaur及Tm4sf1，这些基因可为脊髓损伤的治疗研究提供新的思路。